The Asian Journal of Horticulture

Suhasini Jalawaadi ¹, R. C. Jagadeesha ², D. Kiranshankar ², Kulapati Hipparagi ², G. Prabhuling ², H. R. Basavarajappa ²

Affiliations
1 K.R.C. College of Horticulture, Arabhavi, BBelgaum Karnataka, IN
2 K.R.C. College of Horticulture, Arabhavi, Belgaum Karnataka, IN

Abstract

To study the molecular characterization of 31 accessions of sapota comprising 19 cultivars, 6 land races and 6 hybrids of sapota using PCR based Random Amplified Polymorphic DNA (RAPD) markers. DNA isolated by CTAB method was used for amplification of 48 markers by using 7 RAPD primers. All 48 polymorphic fragments were used to generate the similarity matrix and construct a dendrogram. In this matrix highest genetic similarity of 100 per cent was observed between the 'DHS-1' and 'DHS-2', while least (23 %) was between 'PKM-3' and 'Culcutta Round'. UPGMA (Unweighted Pair Group Method with Arithmetical averages) cluster analysis using Jaccard's co-efficient of similarity of 31 genotypes showed medium to high diversity, which are distributed between the ranges of 35-100 per cent. For the 19 cultivars, the maximum similarity of 91 units was found between 'Murabba' and 'Oval'. 'Pala' and 'Virudhnagar' were closely placed due to 71 per cent oval shaped fruits. Among landraces the round shaped fruit bearing landraces were grouped in cluster-I, and 'Cricket Ball (Udupi)', 'Cricket Ball (Sirsi)' were closely associated with each other as they are having distinctively round shaped fruits and they are 78 per cent similar. Among hybrids 'DHS-1', 'DHS-2', 'PKM-2', CO-1' and 'CO-3' which are grouped together because of their oval and round shaped fruits with spreading and single bearing habit

Keywords

RAPD, Molecular Characterization, Jaccard’s Co-efficient

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A. B. Mastiholi ¹, G. Prabhuling ², S. T. Bhairappanavar ³

Affiliations
1 Kittur Rani Channamma College of Horticulture, Arabhavi, Belgaum Karnataka, IN
2 Kittur Rani Channamma College of Horticulture, Arabhavi, Belgaum Karnataka
3 Department of Agronomy, College of Agriculture, Hassan Karnataka, IN

Abstract

A field investigation to know the effect of spacing and time of harvest on nutrient uptake in medicinal coleus was conducted at Kittur Rani Channamma College of Horticulture, Arabhavi during Kharif 2004 and 2005. Closer spacing of 60 cm x 20 cm recorded significantly higher nutrient uptake (21.6, 2.6 and 25.3 kg / ha of N, P and K, respectively) in tubers. Higher uptake of N, P and K at 160 (18.8, 2.2 and 21.7 kg / ha, respectively) and 180 DAP (17.9, 2.2 and 20.7 kg / ha, respectively) was recorded in tubers. Spacing and time of harvest did not influence the total uptake of nutrients. Higher uptake of NPK (102.1, 11.2 and 117.1 kg / ha, respectively) was recorded at 140 DAP in top portion.

Keywords

Spacing, Time of Harvest, Uptake, Tuber, Biomass

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G. Prabhuling ¹, A. B. Mastiholi ¹, M. G. Kerutagi ¹

Affiliations
1 K.R.C. College of Horticulture, Arabhavi, Belgaum Karnataka, IN

Abstract

Water source in plant tissue culture production laboratories is from distillation units. The costs of these units are a high and regular maintenance is also economically taxing. Hence, to reduce the cost of production of tissue cultured banana, in vitro multiplication and ischolar_maining was carried out on a medium prepared with different sources of water viz., millipore filter water, aquaguard filter water, double distilled water, single distilled water and autoclaved potable tap water. Cultures grown on MS medium prepared with aquaguard filter water recorded maximum mean number of shoots/culture (12.50), highest mean shoot length (2.77 cm) and maximum mean number of adventitious buds/culture (8.25) followed by millipore filter water and autoclaved potable tap water. Microshoots cultured on MS medium prepared with aquaguard filter water recorded highest ischolar_maining percentage (100 %), maximum number of primary ischolar_mains/ shoots (9.50) and highest ischolar_main length (7.00 cm) followed by millipore filter water. Cheaper source of water such as aquaguard filter or even autoclaved potable tap water can be used as low cost alternative water source for successful micropropagation of banana 'Grande Naine'.

Keywords

Aquaguard Filter Water, Grande Naine, Micropropagation, Millipore Filter Water, Potable Tap Water

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